Diverse and Newly Recognized Effects Associated with Short Interfering RNA Binding Site Modifications on the Tomato Bushy Stunt Virus P19 Silencing Suppressor
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چکیده
منابع مشابه
Diverse and newly recognized effects associated with short interfering RNA binding site modifications on the Tomato bushy stunt virus p19 silencing suppressor.
The Tomato bushy stunt virus-encoded P19 forms dimers that bind duplex short interfering RNAs (siRNAs) to suppress RNA silencing. P19 is also involved in multiple host-specific activities, including the elicitation of symptoms, and in local and/or systemic spread. To study the correlation between those various roles and the siRNA binding by P19, predicted siRNA-interacting sites were modified. ...
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The motional state of RNA in tomato bushy stunt rirlls, both in thr crystalline st,attx and in solution, has been invrstigat,ed IuGng 31P nuclear magnetic resonance methods. It, has been found that t’hr RNA is highly immobile in tho nativ Crus. and it is suggested that t,he lack of a high-resolution X-ray diffraction patt,crn for either the RNB or the N-terminal repions of the pro&n coat molcrn...
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Tomato bushy stunt virus particles have previously been physically and chemically well characterized. Different strains, however, are known to have different host plants and symptoms, and they can be distinguished serologically. In an attempt to correlate this behavior with physicochemical properties, we have performed a comparative study on isolates of four of the main variants of this virus (...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 2009
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.02186-08